Matrices listed below were spiked with certain level of TGF-beta1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGF-beta1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-105
100
EDTA plasma(n=5)
87-103
97
heparin plasma(n=5)
97-102
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGF-beta1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TGF-beta1 ELISA Kit allows for the in vitro quantitative determination of TGF-beta1 concentrations in serum, plasma, tissue homogenates and other biological fluids.