Matrices listed below were spiked with certain level of sCD80 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD80 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
92
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
89-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD80 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
sCD80 ELISA Kit allows for the in vitro quantitative determination of sCD80 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat sCD80(Soluble Cluster of Differentiation 8) ELISA Kit