Matrices listed below were spiked with certain level of MMP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
98
EDTA plasma(n=5)
86-101
92
heparin plasma(n=5)
86-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MMP-1 ELISA Kit allows for the in vitro quantitative determination of MMP-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat MMP-1(Matrix Metalloproteinase 1) ELISA Kit