Matrices listed below were spiked with certain level of IL-9 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
98
EDTA plasma(n=5)
88-99
93
heparin plasma(n=5)
89-99
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-9 ELISA Kit allows for the in vitro quantitative determination of IL-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.