Matrices listed below were spiked with certain level of IL-1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-98
95
EDTA plasma(n=5)
87-105
94
heparin plasma(n=5)
87-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-1alpha ELISA Kit allows for the in vitro quantitative determination of IL-1alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat IL-1alpha(Interleukin 1 Alpha) ELISA Kit