Matrices listed below were spiked with certain level of ENA-78 and the recovery rates were calculated by comparing the measured value to the expected amount of ENA-78 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-95
91
EDTA plasma(n=5)
88-105
97
heparin plasma(n=5)
85-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ENA-78 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ENA-78 ELISA Kit allows for the in vitro quantitative determination of ENA-78 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ENA-78(Epithelial Neutrophil Activating Peptide 78) ELISA Kit