Matrices listed below were spiked with certain level of IL-25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
96
EDTA plasma(n=5)
87-94
90
heparin plasma(n=5)
92-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-25 ELISA Kit allows for the in vitro quantitative determination of IL-25 concentrations in serum, plasma, tissue homogenates and other biological fluids.