Matrices listed below were spiked with certain level of ECF/CCL11 and the recovery rates were calculated by comparing the measured value to the expected amount of ECF/CCL11 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
86-105
94
heparin plasma(n=5)
92-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ECF/CCL11 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ECF/CCL11 ELISA Kit allows for the in vitro quantitative determination of ECF/CCL11 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit