Matrices listed below were spiked with certain level of CD59a and the recovery rates were calculated by comparing the measured value to the expected amount of CD59a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
87-100
95
heparin plasma(n=5)
88-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD59a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD59a ELISA Kit allows for the in vitro quantitative determination of CD59a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse CD59a(CD59A glycoprotein) ELISA Kit