Matrices listed below were spiked with certain level of CD47 and the recovery rates were calculated by comparing the measured value to the expected amount of CD47 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-99
97
EDTA plasma(n=5)
86-104
92
heparin plasma(n=5)
87-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD47 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
97-104%
88-105%
85-100%
EDTA plasma(n=5)
88-100%
84-100%
83-97%
heparin plasma(n=5)
80-97%
82-98%
82-95%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q61735
Applications
ELISA
Applications Notes
CD47ELISA Kit allows for the in vitro quantitative determination of CD47 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse CD47(Leukocyte surface antigen CD47)ELISA Kit