Matrices listed below were spiked with certain level of CD3 and the recovery rates were calculated by comparing the measured value to the expected amount of CD3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-95
91
EDTA plasma(n=5)
90-104
97
heparin plasma(n=5)
85-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD3 ELISA Kit allows for the in vitro quantitative determination of CD3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse CD3(Cluster of differentiation 3) ELISA Kit