Matrices listed below were spiked with certain level of TPO and the recovery rates were calculated by comparing the measured value to the expected amount of TPO in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
94
EDTA plasma(n=5)
90-97
93
heparin plasma(n=5)
89-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TPO ELISA Kit allows for the in vitro quantitative determination of TPO concentrations in serum, plasma, tissue homogenates and other biological fluids.