Matrices listed below were spiked with certain level of TNFalpha and the recovery rates were calculated by comparing the measured value to the expected amount of TNFalpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
94
EDTA plasma(n=5)
93-104
99
heparin plasma(n=5)
89-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFalpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TNF-alpha ELISA Kit allows for the in vitro quantitative determination of TNFalpha concentrations in serum, plasma, tissue homogenates and other biological fluids.