Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-97
91
EDTA plasma(n=5)
91-102
98
heparin plasma(n=5)
85-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.