Matrices listed below were spiked with certain level of IL-35 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-35 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
97
EDTA plasma(n=5)
88-104
98
heparin plasma(n=5)
93-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-35 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-35 ELISA Kit allows for the in vitro quantitative determination of IL-35 concentrations in serum, plasma, tissue homogenates and other biological fluids.