Matrices listed below were spiked with certain level of IL-21 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-21 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
95
EDTA plasma(n=5)
85-95
91
heparin plasma(n=5)
85-103
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-21 ELISA Kit allows for the in vitro quantitative determination of IL-21 concentrations in serum, plasma, tissue homogenates and other biological fluids.