Matrices listed below were spiked with certain level of IL-17E/IL-25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-17E/IL-25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
93
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
89-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-17E/IL-25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-17E/IL-25 ELISA Kit allows for the in vitro quantitative determination of IL-17E/IL-25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IL-17E/IL-25(Interleukin 25) ELISA Kit