Matrices listed below were spiked with certain level of IL-17 A/F and the recovery rates were calculated by comparing the measured value to the expected amount of IL-17 A/F in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
95
EDTA plasma(n=5)
93-103
98
heparin plasma(n=5)
85-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-17 A/F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
86-97%
89-101%
EDTA plasma(n=5)
83-96%
82-100%
84-99%
heparin plasma(n=5)
80-100%
80-99%
83-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IL-17 A/F ELISA Kit allows for the in vitro quantitative determination of IL-17 A/F concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IL-17 A/F(Interleukin 17A/F ) ELISA Kit