Matrices listed below were spiked with certain level of IL-12 (p40) and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 (p40) in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-98
93
EDTA plasma(n=5)
85-101
96
heparin plasma(n=5)
85-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 (p40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 (p40) concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IL-12(p40)(Interleukin 12 p40) ELISA Kit