Matrices listed below were spiked with certain level of IFNalpha2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFNalpha2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
97
EDTA plasma(n=5)
88-93
91
heparin plasma(n=5)
86-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNalpha2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-99%
97-104%
88-102%
EDTA plasma(n=5)
85-101%
82-99%
88-99%
heparin plasma(n=5)
83-98%
86-95%
80-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P01563
Applications
ELISA
Applications Notes
IFNalpha2ELISA Kit allows for the in vitro quantitative determination of IFNalpha2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IFNalpha2(Interferon alpha-2)ELISA Kit