Matrices listed below were spiked with certain level of CD80 and the recovery rates were calculated by comparing the measured value to the expected amount of CD80 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-97
94
EDTA plasma(n=5)
95-102
99
heparin plasma(n=5)
88-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD80 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD80 ELISA Kit allows for the in vitro quantitative determination of CD80 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CD80(T-lymphocyte activation antigen CD80) ELISA Kit