Matrices listed below were spiked with certain level of CD27 and the recovery rates were calculated by comparing the measured value to the expected amount of CD27 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
96
EDTA plasma(n=5)
87-103
92
heparin plasma(n=5)
95-100
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
86-105%
89-103%
EDTA plasma(n=5)
84-99%
82-98%
82-99%
heparin plasma(n=5)
81-100%
82-99%
81-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P26842
Antigen
CD27
Applications
ELISA
Applications Notes
CD27 ELISA Kit allows for the in vitro quantitative determination of CD27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CD27(T-cell activation antigen CD27) ELISA Kit