Matrices listed below were spiked with certain level of CCL25 and the recovery rates were calculated by comparing the measured value to the expected amount of CCL25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
85-97
90
heparin plasma(n=5)
90-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CCL25 ELISA Kit allows for the in vitro quantitative determination of CCL25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CCL25(C-C motif chemokine 25) ELISA Kit