Matrices listed below were spiked with certain level of MIP-3beta and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-3beta in samples.<br /><br /><table><thead class="border-blue-600 border-b-2 text-left"><tr><th class="p-2">Matrix</th><th class="p-2">Recovery range(%)</th><th class="p-2">Average(%)</th></tr></thead> <tbody><tr><td class="p-2">serum(n=5)</td><td class="p-2">88-99</td><td class="p-2">94</td></tr><tr><td class="p-2">EDTA plasma(n=5)</td><td class="p-2">89-101</td><td class="p-2">94</td></tr><tr><td class="p-2">heparin plasma(n=5)</td><td class="p-2">89-103</td><td class="p-2">95</td></tr></tbody></table>
Linearity :
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-3beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.<br /><br /><table><thead class="border-blue-600 border-b-2 text-left"><tr><th class="p-2">Sample</th><th class="p-2">1:2</th><th class="p-2">1:4</th><th class="p-2">1:8</th></tr></thead> <tbody><tr><td class="p-2">serum(n=5)</td><td class="p-2">87-105%</td><td class="p-2">93-101%</td><td class="p-2">85-104%</td></tr><tr><td class="p-2">EDTA plasma(n=5)</td><td class="p-2">83-97%</td><td class="p-2">86-96%</td><td class="p-2">84-98%</td></tr><tr><td class="p-2">heparin plasma(n=5)</td><td class="p-2">87-98%</td><td class="p-2">80-95%</td><td class="p-2">83-97%</td></tr></tbody></table>
MIP-3beta ELISA Kit allows for the in vitro quantitative determination of MIP-3beta concentrations in serum, plasma, tissue homogenates and other biological fluids.