• Clonality :
• Monoclonal

• Description :
• TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

• Formulation :
• 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Host :
• Mouse

• Immunogen Region :
• A recombinant fragment of human TIM3 protein within amino acids 22-202 was used as the immunogen for the TIM3 antibody.

• Isotype :
• IgG2a,kappa

• Predicted Reactivity :
• Human

• Reactivity :
• Human

• Recombinant :
• No

• Subcellular Location :
• Cell surface, cytoplasmic

• Antigen :
• TIM-3

• Uniprot :
• Q8TDQ0

• Clone No :
• TIM3/3113

• Concentration :
• 0.2 mg/ml

• Format :
• Purified

• Purification :
• Protein G affinity chromatography

• Storage :
• Store the TIM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

• Product Manual :
• <a href="http://www.nsjbio.com/tds-pdf/tim3-antibody-tim3-3113-protein-microarray-validated-v7582" target="_blank">Data Sheet</a>