• Alternative Names :
• Cell cycle checkpoint protein RAD1 (hRAD1) (EC 3.1.11.2) (DNA repair exonuclease rad1 homolog) (Rad1-like DNA damage checkpoint protein), RAD1, REC1

• Categories :
• Primary Antibodies

• Clonality :
• polyclonal

• Description :
• Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

• Host :
• Rabbit

• Immunogen :
• Recombinant Human Cell cycle checkpoint protein RAD1 protein (1-282AA)

• Isotype :
• IgG

• Raised In :
• Rabbit

• Reactivity :
• Human

• Regulatory :
• RUO

• Relevance :
• Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

• Species :
• Homo Sapiens (Human)

• Subcellular Location :
• Nucleus

• Uniprot :
• O60671

• Database Links :
• 
  

  HGNC:9806

  OMIM:603153

  KEGG:hsa:5810

  STRING:9606.ENSP00000340879

  UniGene:Hs.38114

• Function :
• Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair

• Pathway :
• Cellular senescence

• Protein Families :
• Rad1 family

• Research Area :
• Epigenetics and Nuclear Signaling

• Tissue Specificity :
• Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).

• Buffer :
• PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Form :
• Liquid

• Format :
• Liquid

• Purification :
• Antigen Affinity Purified

• Purity :
• Antigen Affinity Purified

• Storage :
• Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

• Storage Buffer :
• 
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Datasheet :
• 

• ELISA Protocol :

• Western Blotting(WB) Protocol :

• Immunohistochemistry (IHC) Protocol :