ZDF Spleen Endothelial Cells

Mfr.No.	RD-6057
Description	The Zucker Diabetic Fatty (ZDF) Rat Primary Spleen Endothelial Cells from Cell Biologics are isolated from the spleen tissues of 9-week old ZDF Rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. ZDF Rat Primary Spleen Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Type	Endothelial
Cell Models	ZDF
Media	M1266 Complete Endothelial Cell Medium w/ Kit - 500 ml
Tissue	Spleen
Species	Rat
Note	Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Product Use	ZDF Rat Primary Spleen Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16405088	RD-6057	Frozen Vial	-		Add to Quote