UM-HACC-2A cells were developed from a human with a T3N1M0 ACC in their minor salivary gland. The cell line is transplantable orthopedically in the submandibular gland and is metastatic. The cells are resistant to cisplatin. The UM-HACC-2A cell lines may be used for mechanistic investigations and developmental therapeutics studies.
Disease
T3N1M0 ACC in a minor salivary gland at the base of the tongue
Density
10,000 cells/cm2
Ethnicity
Caucasian
Growth Properties
148
Quantity
1x106 cells / 1.0 ml
Morphology
Epithelial
Organism
Human
Propagation
Fibronectin Coating Solution (1 mg/mL) (TM059) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 2 mM L-glutamine (G275) + 0.4 ?g/ml Hydrocortisone + 20 ng/ml EGF (Z100139) + 5 ?g/ml insulin (TM053) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?
Markers
MYB:NFIB fusion (detected via RT-PCR), resistant to cisplatin
Source Organ
Salivary Gland
Shipping
Dry Ice
Storage
-180°C
Reference
Warner, K. A., Oklejas, A. E., Pearson, A. T., Zhang, Z., Wu, W., Divi, V., . . . Nör, J. E. (2018). UM-HACC-2A: MYB-NFIB fusion-positive human adenoid cystic carcinoma cell line. Oral Oncology, 87, 21-28. doi:10.1016/j.oraloncology.2018.10.012