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Primary Minipig (Gottingen) Hepatocytes, Suspension

SKU : BHC10901828

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0113
Description	These cryoplateable hepatocytes are suitable for induction, metabolism and toxicity studies.
Cell Type	Primary Cells
Biosafety Level	2
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Disease	Normal
Donor History	Normal tissue
Growth Properties	Suspension, round
Growth Conditions	PriCoat?T25 Flasks (G299)?re recommended for optimal cell culture.?riGrow X Series Medium (TM0113) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?, saturating humidity.Note: cells will attach to collagen coated plates for 5 days.
Quantity	5x10⁵ cells / 1.0 ml
Tissue	Liver
Morphology	Spherical
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Pig (Porcine)
Species	Minipig
Propagation	PriGrow X Series Medium (TM0113) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Cryopreservation	abm?oes not recommend cryopreserving cells.
Source Organ	Liver

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 50ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Gently resuspend cells by inverting the tube several times.?br />4. Perform a total cell count and dilute cells to 7.0 x105 cells/ml with complete media. 5. Add the apropriate volume of diluted cells to collagen-coated tissues culture plates or flasks, as desired.?br />6. Incubate the cells at the recommended conditions. 7. After 2-4 hours (after cell attachment has been observed), wash cells with complete medium.?ecommended volumes for plating cells:6-well plate: 2.5 ml/well12-well plate: 1.0?l/well24-well plate: 0.5?l/well48-well plate: 0.2?l/well96-well plate: 70?l/wellT-flasks: 0.25 ml/cm2
Subculture Protocol	abm?oes not recommend subculturing this cell line. We advise using cells for experimental purposes as soon as possible.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10901828	T0113	5x10^5 cells / 1.0 ml	-		Add to Quote

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