Primary Normal Human Kidney Proximal Tubule (NhKPT) cells are isolated by positive magnetic selection from a heterogeneous population of cells taken from the cortical part of the kidney after cortex digestion. NhKPT cells are an integral part of the nephron. The proximal tubule regulates the pH of the filtrate by exchanging hydrogen ions for bicarbonate ions, and it is responsible for secreting organic acids, such as creatinine and other bases, into the filtrate. Primary NhKPT cells have been used to research function and pathophysiology of the kidney, toxicity, ion channels, kidney disease progression and more.
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Morphology
Epithelial-like
Organism
Homo sapiens
Species
Human
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5520) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Source Organ
Kidney
Shipping
Dry Ice
Storage
-180°C
Reviews of Normal Human Primary Kidney Proximal Tubule Cells