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Home / Cells / Primary Cells

Mouse Tumor-Associated Fibroblasts (Hu. Colon Cancer Origin, HCT116)

SKU : BHC16400294

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Cell Biologics, Inc.

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Mfr.No.	MC-6231h
Description	Mouse Tumor-Associated Fibroblasts (Human Colon Cancer Origin, MTCLFb.H) from Cell Biologics were isolated from human xenograft of pathogen-free laboratory nude mice. Human colon adenocarcinoma cells (HCT116) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTCLFb.H were cultured in a T75 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	MTCLFb.H can be used in assays of standard biochemical procedures performed with cell cultures include cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	MTCLFb.H are characterized by immunofluorescent staining with antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics.
Tissue	Colon

Product Use	MTCLFb.H from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400294	MC-6231h	Frozen Vial	$510		Add to Cart

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