The human malignant glioma cell line (SF295) was selected in increasing concentrations of mitoxantrone to yield these cell lines. Cells were maintained in constant culture with mitoxantrone for 3 months before cytogenetic analysis was conducted. MX# indicates the mitoxantrone concentration that the cell line is resistant to in nmol/L. Northern blot analysis was used to confirm ABCG2 expresion in mitoxantrone selected SF295 sublines. The cell lines show increasing ABCG2 expression at the RNA level with increasing mitoxantrone concentration. These cell lines are suitable for studies investigating drug resistance in clinical oncology.
Density
Recommended split ratio 1:10
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Organism
Human (H. sapiens)
Markers
ABCG2
Source Organ
Brain
Shipping
Dry Ice
Storage
-180°C
Reviews of Mitoxantrone-Resistant SF295 Cell Line (MX500)