mIL5 Cells are derived from primary tumors of the intermediate lobe from POMC-Tag transgenic mice. The cells exhibit a branched cytoplasmic extensions in culture, and have large polymorphic nuclei in appearance in contrast to the mIL39 Cells (abm, T8007). There is expression of POMC, but no SV40 Tag expression was observed indicating spontaneous mutation may have occurred. However, the cells are transformed due to the characteristics of fast doubling time and ability to be tumorigenic. mIL5 Cells are able to secrete PRF (PRL-regulating factor), and may be used to study the function of PRL feedback and regulation for the process of lactation.
Cell Type
Tumor Cells
Disclaimer
1. For for-profit organizations and corporations, please contact orders@biohippo.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity
1x106 cells/1.0ml
Tissue
Brain
Morphology
Polygonal
Population Doubling
Doubling time of 15 hours
Species
Mouse (M. musculus)
Propagation
Use of PriCoatTMT25Flasks (G299)orApplied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coatedculturevessels unless otherwise specified in the Propagation Requirements below.The base medium for this cellline isPrigrow II medium available at abm, Cat. No. TM002. To makethe complete growth medium, addthefollowing components to the base medium: fetal bovine serum (TM999) to finalconcentration of 10% and Penicillin/Streptomycin Solution (G255) at a final concentration of1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.