Human Primary Monocyte-derived Dendritic Cells are derived from peripheral blood monocytes, in which the monocytes are differentiated into dendritic cells. Cells do not proliferate in vitro and should be immediately be used for intended application.
Cell Type
Primary Cells
Disclaimer
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.2. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a replacement is possible at a cost (plus shipping).3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
Gender
Donor Info Not Disclosed
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Blood
Morphology
Multipolar
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM5403. Cells do not proliferate for long term culture in vitro. Recommend for immediate use for downstream applications. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
1. Remove and discard culture medium.2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.3. Centrifuge cells at 1500rpm for 3 minutes to pellet.4. Aspirate out trypsin, leaving pellet undisturbed.5. Resuspend pellet in fresh culture medium and plate in new culture vessel.6. Incubate cultures at 37°C.
Reviews of Human Primary Total Liver Cell Population