Human Primary Normal Placental Microvascular Endothelial Cells are isolated from normal human placental tissue. The cells exhibit a cobblestone-like morphology with dark nuclei. They exhibit expression of CD31, and/or VE-Cadherin endothelial cell markers. A valuable tool for cell to cell adhesion assays, or for downstream processes such as RT-PCR or Western blotting for research applications.
Cell Type
Primary Cells
Gender
Donor Info Not Disclosed
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Reproductive
Morphology
Cobble-stone
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM104. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
1. Remove and discard culture medium.2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37
Reviews of Human Primary Placental Epithelial Cells