The Human Primary Mesothelial cells are isolated from the human mesothelium, an important structure lining human abdomen cavities to provide lubrication, transportation and movement of fluids, immune cells, cytokines and proteins. These specialized cells are critical in regulating inflammatory responses and innate immune systems to protect against infections and tumor propagation. Their roles in cancer metastasis, metabolic diseases, peritoneal dialysis and surgical adhesions make them an ideal model for cancer research, pathological and/or therapeutic study.
Cell Type
Primary Cells
Disclaimer
The Human Primary Mesothelial cells are isolated from the human mesothelium, an important structure lining human abdomen cavities to provide lubrication, transportation and movement of fluids, immune cells, cytokines and proteins. These specialized cells are critical in regulating inflammatory responses and innate immune systems to protect against infections and tumor propagation. Their roles in cancer metastasis, metabolic diseases, peritoneal dialysis and surgical adhesions make them an ideal model for cancer research, pathological and/or therapeutic study.
Disease
Normal
Gender
Female
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Peritoneum
Morphology
Epithelial, Fibroblast-like
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4067. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
Volumes are for T25 (or 60mm dish)1. Coat a flask with gelatin by adding 3-5ml sterile gelatin solution to a T25 and incubate for 30 minutes. Flasks may be stored at 4C. Aspriate gelatin solution immediately before use2. Aspirate the medium from the stock culture and rinse with 3ml Versene3. Add 1ml 0.05% trypsin-0.02% EDTA4. Incubate the flask at 37C until cells round up (1-5 minutes)5. Add 5ml of growth medium to the flask and dislodge any undetached cells by gently pipetting the medium against the bottom surface of the flask.6. Triturate gently to make a single-cell suspension and transfer suspension to a 15ml centrifuge tube7. Remove a 0.5ml aliquot to count the cells8. Centrifuge at 100 x g for 5-10 minutes9. Aspitate supernatant, flick tube to break up pellet, and resuspend cells in growth medium10. For stocks, generally plate T25 or T75 flasks with 4,000-10,000 cells/cm^2