Human Primary Normal Kidney Endothelial Cells are isolated from normal human kidney tissue. The cells exhibit a cobblestone-like morphology with dark nuclei. They exhibit expression of CD31, and/or VE-Cadherin endothelial cell markers. A valuable tool for cell to cell adhesion assays, or for downstream processes such as RT-PCR or Western blotting fpr research applications.
Cell Type
Primary Cells
Disclaimer
CD31, VE-Cadherin
Gender
Donor Info Not Disclosed
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Renal
Morphology
Cobble-stone
Species
Human (H. sapiens)
Propagation
The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM104. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
1. Remove and discard culture medium.2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.3. Centrifuge cells at 1500rpm for 3 minutes to pellet.4. Aspirate out trypsin, leaving pellet undisturbed.5. Resuspend pellet in fresh culture medium and plate in new culture vessel.6. Incubate cultures at 37°C.
Reviews of Human Primary Intra-Hepatic Biliary Epithelial Cells