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Human Primary Dermal Epithelial Cells

SKU : BHC16400032

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Cell Biologics, Inc.

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Mfr.No.	H-6066
Description	Human Primary Dermal Epithelial Cells from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Human Primary Dermal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Human
Cell Type	Epithelial Cells
Application	Human Primary Dermal Epidermal Keratinocytes can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Although Human Primary Corneal Epithelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	Human Primary Dermal Epidermal Keratinocytes from Cell Biologics are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Media	Complete Epithelial Cell Medium w/ Kit 500 ml
Tissue	Dermal
Notes	Investigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Species	Human

Applications	Dermatologic Research

Product Use	Human Primary dermal Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400032	H-6066	0.5x106 Cells	$660		Add to Cart

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