The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM104. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
1. Remove and discard culture medium.2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.3. Centrifuge cells at 1500rpm for 3 minutes to pellet.4. Aspirate out trypsin, leaving pellet undisturbed.5. Resuspend pellet in fresh culture medium and plate in new culture vessel.6. Incubate cultures at 37°C.
Reviews of Human Primary Colon Smooth Muscle Cells