Chondrocytes in vivo produce and maintain the extracellular matrix of the cartilage. In vitro, chondrocytes after serial passages as a monolayer culture have been documented to lose their phenotypic markers and become de-differentiated. Re-differentiation can be induced by culturing the cells in a 3-D culture system. These cells are useful in studying chondrocyte differentiation and effects of cytokines and growth factors on the maintenance of differentiated phenotype.
Cell Type
Primary Cells
Disclaimer
Chondrocytes in vivo produce and maintain the extracellular matrix of the cartilage. In vitro, chondrocytes after serial passages as a monolayer culture have been documented to lose their phenotypic markers and become de-differentiated. Re-differentiation can be induced by culturing the cells in a 3-D culture system. These cells are useful in studying chondrocyte differentiation and effects of cytokines and growth factors on the maintenance of differentiated phenotype.
Disease
Normal
Gender
Donor Info Not Disclosed
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Skeletal
Morphology
Multipolar
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is abm, Cat. No. TM0020.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.