JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cells / Primary Cells

Cynomolgus Monkey Primary Retinal Microvascular Endothelial Cells

SKU : BHC16400167

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Cell Biologics, Inc.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	MK-6065
Description	Monkey Primary Retinal Microvascular Endothelial Cells from Cell Biologics are isolated from Cynomolgus Monkey retinal tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Monkey Primary Retinal Microvascular Endothelial are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. ab9498, Abcam), VE-cadherin (Catalog No. sc-28644, Santa Cruz, or Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. Monkey Primary Retinal Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.
Cell Origin	Cynomolgus Monkey
Cell Type	Endothelial Cells
Application	Cynomolgus Monkey Primary Retinal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Although Monkey Primary Retinal Microvascular Endothelial Cells are isolated from normal monkey tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	Cynomolgus Monkey Primary Retinal Endothelial Cells from Cell Biologics display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for LDL uptake positive by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded on a plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Media	Complete Monkey Endothelial Cell Medium 125 ml
Tissue	Retinal Microvascular
Notes	Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at
Species	Monkey

Applications	Ophthalmology Research

Product Use	Monkey Primary Retinal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHC16400167	MK-6065	0.5x106 Cells	$860		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.