CV-1 cells were transfomed with an origin-defective mutant of SV40 coding for the wild-type T antigen. The cell line retains complete permissiveness for lytic growth of SV40 mutants with deletions in the early region and support the replication of pure populations of SV40 mutants.
Density
10,000 cells/cm2; Recommended split ratio 1:4 to 1:8