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CD1 Mouse Hepatic Stellate Cells

SKU : BHC16400737

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Cell Biologics, Inc.

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Mfr.No.	CD-1242
Description	CD1 Mouse Primary Hepatic Stellate Cells from Cell Biologics are isolated from liver tissues of pathogen-free laboratory CD1 mice and grown in tissue culture plates with Cell Biologics Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 0.2x10^6 cells. Vitamin A droplets in Hepatic Stellate Cells are visualized by blue autofluorescence when excited with UV light. These cells can be used for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommended for long-term cultures since these cells would differentiate into fibroblat-like cells after plating, and cells will not proliferate in culture and can not be passaged.
Cell Origin	Mouse
Cell Type	Hepatic Stellate Cells
Application	CD1 Mouse Primary Hepatic Stellate Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Freeze Thaw Recovery	Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.Promptly remove the vial and wipe it down with 70% ethanol.Transfer cells from the vial to a sterile centrifuge tube. Add 5 ml of pre-warmed Cell Biologics Cell Culture Medium. Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.Centrifuge cells at 200 g for 5 minutes.Aspirate the supernatant and resuspend the cell pellet in 3 ml of Cell Biologics Cell Culture Growth medium.Add resuspended cells into a tissue-culture treated plate (to seed on 2-3 wells of a 12-well plate or 1 well of 6-well plate).Place a plate in a humidified, 5%-CO2 incubator at 37°C until experiments.Change fresh cell culture medium every 24-48 hours.Cells should be checked daily under a microscopy to verify appropriate cell morphology.Note
Frozen	0.5×10⁶cells
General Information	CD1 Mouse Primary Hepatic Stellate Cells from Cell Biologics are tested for expression of markers using antibodies, Desmin or ?-actin by Immunofluorescence Staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommend for expanding or long term cultures since these cells would differentiate to become fibroblat-like cells immediately after plating and they will not proliferate in culture.
Media	Complete Hepatic Stellate Cell Medium /w Kit (500 ml)
Tissue	Liver
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse
Species details	CD1 Mouse

Applications	Hepatologic Research

Product Use	CD1 Mouse Primary Hepatic Stellate Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400737	CD-1242	0.2x106 Cells	$560		Add to Cart

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