Canine Primary Thymus Epithelial Cells from Cell Biologics are isolated from thymus tissue of beagle dog. Canine Primary Thymus Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium for 3-5 days. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods.Canine Primary Thymus Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Cell Origin
Canine
Cell Type
Epithelial Cells
Frozen
0.5-1×106cells
Tissue
Uterine
Reviews of Canine Primary Uterine Epithelial Cells