C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias of pathogen-free laboratory adult mice. Cells are grown in tissue culture plates with Cell Biologics Culture Medium for 7-15 days. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x106 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44, Sca-1 or CD29 by flow cytometry.
Cell Origin
Mouse
Cell Type
Mesenchymal Stem Cells
Application
Mouse Bone Marrow Mesenchymal Stem Cells can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, or cell derivatives for desired research applications.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen
2×106cells
Tissue
Bone
Product Use
Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.
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