This breast tumor line is derived from a 74-year-old, human caucasian female with breast cancer. Cell proliferation is inhibited by TNG-alpha and the BT-20 cells are negative for estrogen receptor. However, they express an estrogen receptor mRNA with a deletion of exon 5. This cell line tumorigenic in nude mice abd forms grade II adenocarcinomas.
Density
10,000 cells/cm2; Recommended split ratio 1:2 to 1:4