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BALB/c Mouse Primary Lung Microvascular Endothelial Cells

SKU : BHC16400347

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Cell Biologics, Inc.

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Mfr.No.	BALB-5011
Description	BALB/c Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. BALB/c Mouse Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R&D System) or CD31/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Mouse
Cell Type	Endothelial Cells
Application	BALB/c Mouse Primary Lung Microvascular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although BALB/c Mouse Primary Lung Microvascular Endothelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	BALB/c Mouse Primary Lung Microvascular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. BALB/c Mouse Primary Lung Microvascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Media	Complete Endothelial Cell Medium w/ Kit 500 ml
Tissue	Lung Microvascular
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse
Species details	BALB/c Mouse

Applications	Pulmonary Research

Product Use	BALB/c Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400347	BALB-5011	0.5x106 Cells	$590		Add to Cart

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