al458-c cell line is derived from advanced non-necrotic murine liver tumors generated after transposon/CRISPR vector delivery to induce stable oncogene expression. Part of the mCL1 subclass of hepatocellular carcinoma (HCC), the cells expresses epithelial features and expression of hepatocyte and liver fetal/progenitor markers.The novel collection of the 14 HCC mouse cell lines are available at abm, enabling end users the ability to conduct in depth characterization of gene function, elucidation of molecular mechanisms, and preclinical testing for HCC.
Cell Type
Tumor Cells
Disclaimer
1. For for-profit organizations and corporations, please contact orders@biohippo.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period.
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Tissue
Liver
Morphology
Epithelial
Population Doubling
48-72 hours
Species
Mouse (M. musculus)
Propagation
Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?. No known drug resistance.