Tau 2N Antibody

Mfr.No.	SPC-813
Immunogen Species	Human
Alternative Names	Tau, 2N4R Tau, 2N3R Tau, pTau, MAPT, Neurofibrillary tangle protein, Tau-Human
Available Conjugates	APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Background	Tau is a microtubule-associated protein (MAPT) with multiple isoforms due to alteative mRNA splicing (1,2). The 2N isoform contains two N-terminal inserts and is expressed in both 3R (2N3R) and 4R (2N4R) variants (2). The 3R/4R ratio varies across diseases; for example, Alzheimer’s disease features a mixed 3R/4R pathology (3). Our human Tau 2N3R/4R polyclonal antibody has been demonstrated to be specific to only human 2N isoforms and does not detect rodent isoforms.
Cellular Localization	Plasma Membrane
Certificate Of Analysis	A 1:1000 dilution of SPC-813 was sufficient for detection of Tau 2N3R/4R spliceforms (range tested 2ug – 0.063ug).
Clonality	Polyclonal
Description	Rabbit Anti-Human Tau Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Immunogen Region	Tau 2N3R/4R peptide
Recommended Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.
Shipping Temperature	Blue Ice or 4ºC
Species Reactivity	Human
Specificity	Detects Tau 2N3R/4R spliceforms

Research Area	ALS Disease, Alzheimer's Disease, Neurodegeneration, Parkinson's Disease, Neuroscience

Concentration	1mg/mL
Purification	Affinity Purified
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Storage Temperature	-20ºC, Conjugated antibodies should be stored according to the product label

Applications	WB

Reference	1.,Goedert, M., Spillantini, M. G., Jakes, R., Rutherford, D., & Crowther, R. A. (1989). Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron, 3(4), 519–526. DOI: 10.1016/0896-6273(89)90210-9 2.,Spillantini, M. G., & Goedert, M. (2013). Tau pathology and neurodegeneration. The Lancet Neurology, 12(6), 609–622. DOI: 10.1016/S1474-4422(13)70090-5 3.,Kovacs, G. G. (2017). Tauopathies. Handbook of Clinical Neurology, 145, 355–368. DOI: 10.1016/B978-0-12-802395-2.00025-3

Dot blot analysis comparing detection of Tau isoforms. Of note is that SPC-813 does not bind to 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

Western blot analysis comparing detection of Tau isoforms, human Tau 0N3R monomer (SPR-490), human Tau 1N3R monomer (SPR-513), and human Tau 2N3R monomer. Of note is that SPC-813 does not bind to the 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

Dot blot analysis comparing detection of mouse Tau 2N4R monomer and human Tau 2N4R pre-formed fibrils. Of note is that SPC-813 does not bind to the mouse construct. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11933477-1	SPC-813S	Unconjugated	12 ug	$45	Add to Cart
BHA11933477-2	SPC-813D	Unconjugated	100 ug	$385	Add to Cart
BHA11933477-3	SPC-813D-APC	APC	100 ug	$432	Add to Cart
BHA11933477-4	SPC-813D-A390	ATTO 390	100 ug	$434	Add to Cart
BHA11933477-5	SPC-813D-A488	ATTO 488	100 ug	$433	Add to Cart
BHA11933477-6	SPC-813D-A594	ATTO 594	100 ug	$433	Add to Cart
BHA11933477-7	SPC-813D-BI	Biotin	100 ug	$429	Add to Cart
BHA11933477-8	SPC-813D-FITC	FITC	100 ug	$425	Add to Cart
BHA11933477-9	SPC-813D-HRP	HRP	100 ug	$421	Add to Cart
BHA11933477-10	SPC-813D-HRP	PerCP	100 ug	$432	Add to Cart
BHA11933477-11	SPC-813D-RPE	RPE	100 ug	$430	Add to Cart

Tau 2N Antibody

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