SARS-CoV-1 monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag technology. Biotinylation is confirmed to be>=90%. VHHs are single-domain antibodies from camelids.
assay conditions: Spike Trimer (S1+S2), His-tag (SARS-CoV-1) (BPS Bioscience, #100789) and Spike S1 Neutralizing Antibody (VHH), Fc-fusion (IgG1), Avi-Tag (SARS-CoV-1),Biotin-labeled binding was measured by ELISA using Strepavidin-HRP and Colorimetric HRP substrate.
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.